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KMID : 0385920110220040335
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Journal of the Korean Society of Emergency Medicine
2011 Volume.22 No. 4 p.335 ~ p.342
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Effect of Calcium Channel Blocker on Primary Rat Cardiomyoblasts and Cardiac Rate in Vitro
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park Young-Min
Hwang Yong
Cho Tae-Eun
Yoo Su-Jin
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Abstract
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Purpose: This work was intended to establish experimental conditions for monitoring the effect of ischemic/reperfusion injury on the beating capability of, and apoptotic damage to, primary rat cardiomyoblasts, and to investigate the cardioprotective effect of calcium channel blocker on primary rat cardiomyoblasts with ischemic/reperfusion injury including reactive oxygen species damage.
Methods: To generate ischemic/reperfusion injury, primary rat cardiomyoblasts were treated with hydrogen peroxide (H2O2). Injured cardiomyoblasts were pretreated with the calcium channel blocker nicardipine.
Results: Treatment with H2O2 significantly decreased the cardiac rate of primary rat cardiomyoblasts in time- and dose-dependent manners. Interestingly, the cardiac rate of primary rat cardiomyoblasts abruptly dropped prior to the decrease in cell viability. H2O2 also induced a decrease in the expression of heme oxygenase-1 (HO-1) protein in P2 primary rat cardiomyoblasts in a time-dependent manner. Moreover, treatment with H2O2 resulted in an increase of DNA fragmentation, indicating that H2O2 induced the apoptotic death of P2 primary rat cardiomyoblasts. However, pretreatment with nicardipine markedly decreased cell death in H2O2-treated primary rat cardiomyoblasts by regulating HO-1 protein expression.
Conclusion: Nicardipine has a cardioprotective effect that helps maintain the viability of primary rat cardiomyoblasts with induced ischemic/reperfusion injury, including reactive oxygen species-induced damage.
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KEYWORD
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Calcium channel blocker, Hydrogen peroxide, Apoptosis, Ischemia
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